منابع مشابه
Techniques and applications of NMR to membrane proteins.
The fact that membrane proteins are notoriously difficult to analyse using standard protocols for atomic-resolution structure determination methods have motivated adaptation of these techniques to membrane protein studies as well as development of new technologies. With this motivation, liquid-state nuclear magnetic resonance (NMR) has recently been used with success for studies of peptides and...
متن کاملRecent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins.
In the last 15 years substantial advances have been made to place isotope labels in native and glycosylated proteins for NMR studies and structure determination. Key developments include segmental isotope labeling using Native Chemical Ligation, Expressed Protein Ligation and Protein Trans-Splicing. These advances are pushing the size limit of NMR spectroscopy further making larger proteins acc...
متن کاملHigh-resolution NMR spectroscopy of membrane proteins in aligned bicelles.
High-resolution solid-state NMR spectra can be obtained from uniformly (15)N-labeled membrane proteins in magnetically aligned bicelles. Fast uniaxial diffusion about the axis of the bilayer normal results in single-line spectra that contain the orientational information necessary for protein structure determination.
متن کاملStructure determination of membrane proteins by NMR spectroscopy.
Current strategies for determining the structures of membrane proteins in lipid environments by NMR spectroscopy rely on the anisotropy of nuclear spin interactions, which are experimentally accessible through experiments performed on weakly and completely aligned samples. Importantly, the anisotropy of nuclear spin interactions results in a mapping of structure to the resonance frequencies and...
متن کاملProtein ligation: applications in NMR studies of proteins.
Abbreviations: Abl, Abelson protein tyrosine kinase; CBD, chitin-binding domain; EPL, expressed protein ligation; GPCR, G-protein-coupled receptor; IPL, intein-mediated protein ligation; MBP, maltosebinding protein; NCL, native chemical ligation; NMR, Nuclear Magnetic Resonance; Npu, Nostoc punctiforme; PDB, Protein Data Bank; PTB, polypyrimidine tract binding protein; SH2 and SH3, src homology...
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ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 2017
ISSN: 0003-9861
DOI: 10.1016/j.abb.2017.05.011